Improved fucoxanthin and docosahexaenoic acid productivities of a sorted self-settling Tisochrysis lutea phenotype at pilot scale

This work aimed to select a Tisochrysis lutea phenotype with higher biomass and fucoxanthin productivities using fluorescence-activated cell sorting (FACS). A novel was obtained after 2 rounds of selection, based on high-fucoxanthin fluorescence. The resulting forms aggregates, has no flagella, stable 15 months. Optimal temperature (30 °C) light (300 µmol m?2 s?1) were at laboratory scale, identical the original strain. productivity than strain: 1.9× scale (0.4 L), 4.5× under outdoor conditions (190 L). Moreover, compared strain, increased 1.6–3.1× docosahexaenoic acid 1.5–1.9×. These are highest ever reported productivities, robust new from T. monoculture isolated FACS without genetic manipulation. shows high potential for industrial production.